Cat. #153733
Human AGS NOD1 KO (Clone 41A8) Cell Line
Cat. #: 153733
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Gastric
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Richard Ferrero
Institute: Hudson Institute of Medical Research
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Human AGS NOD1 KO (Clone 41A8) Cell Line
- Alternate name: Human nucleotide-binding oligomerization domain-containing protein 1, NOD1.
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Gastric Adenocarcinoma
- Research fields: Cancer;Cell signaling and signal transduction;Immunology
- Tool sub type: Continuous
- Parental cell: AGS
- Organism: Human
- Tissue: Gastric
- Disease: Cancer
- Growth properties: Epithelial-like morphology
- Model: Knock-Out
- Conditional: No
- Description: NOD1 responds to Helicobacter pylori infection in a Type IV secretion system-dependent manner. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 (as generated by CRISPR) can be used to model gastric adenocarcinoma in the context of various pathogens. NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells.
- Production details: CRISPR/Cas9 gene editing was used to generate NOD1 knock out clones. AGS cells were transfected with vectors expressing ÄË?Â?ÂCas9 nickaseÄË?Â? nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. Clone 41A8 was confirmed as having a 71 base pair deletion within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1. Recommended Freezing Composition: 90% (v/v) FCS - 10% (v/v) DMSO
- Biosafety level: 1
- Additional notes: Recommended Freezing Composition: 90% (v/v) FCS - 10% (v/v) DMSO CRISPR edited Human AGS cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CR...
- Recommended controls: NOD1 Ligand: C12-iE-DAP (10 ng/ml)
Target Details
- Target: Human nucleotide-binding oligomerization domain-containing protein 1
Applications
- Application notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to comp...
Handling
- Format: Frozen
- Growth medium: RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: Human AGS NOD1 KO (Clone 41H8) Cell Line ; Human AGS NOD1 KD (AGS cl.1) Cell Line
References
- Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.
- NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.
