TR146 sc1E3

Launch price: save 28% on both TR146 subclones. Use code TR146LAUNCH at checkout.

Contributors

Inventor Institute
Winfried Neuhaus Austrian Institute of Technology
SKU: 162447
Product description: The TR146 cell line, derived from a squamous cell carcinoma of the buccal mucosa, is an established in vitro model of human oral epithelium and epithelial barriers such as the blood-saliva barrier. Because tumour-derived lines can be heterogeneous, barrier integrity can vary between cultures. TR146 sc1E3 is a single-cell-derived subclone generated to address this. In transwell assays at an air-liquid interface, TR146 sc1E3 formed a tighter epithelial barrier than the parental TR146 line, reaching a peak transepithelial electrical resistance (TEER) of 625.63 ± 93.68 Ω·cm² compared with 137.2 ± 27.24 Ω·cm² for the parental line (mean ± SD, n=3). This was accompanied by reduced transport of 5(6)-carboxyfluorescein across the epithelial layer, consistent with lower paracellular permeability.
Alternate name: TR146 subclone 1E3
Gender: Female
CRISPR: No
Conditional: Yes
Additional notes: For air liquid transwell assays investigating barrier properties cells should be cultured in complete DMEM supplemented with Human Keratinocytes Growth Supplement
Cat. #: 162447
Tool sub type: Continuous
Cancer types: Head and neck cancer
Research Fields: Cancer
Organism: Human
Gender: Female
Morphology: Epithelial
Growth properties: Adherent
Format: Frozen
Growth medium: Dulbecco’s Modified Eagle Medium supplemented with 10 % FCS and 1 % Pen/Strep in a humidified incubator at 37 °C, 5 % CO2.
Mycoplasma free: Yes
Biosafety level: 1
References:

Lin GC. 2023. Establishment and validation of in vitro models of the blood-saliva barrier. Doctoral dissertation, University of Natural Resources and Life Sciences (BOKU), Vienna.

Images

View Gallery
Microscopy image of TR146 sc1E3 cells growing as a confluent monolayer.

Related Tools for Cell lines

Cat. # Tool Name
162139 GL261-NLuc cell line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.