HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line

The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP.

Contributors

Inventor Institute
Francis Barr University of Liverpool
SKU: 152987
Product description: The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP.
Parental cell line: HeLa S3
Cat. #: 152987
Provenance and Ownership: The HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line is owned by Cancer Research UK. CancerTools supplies the HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Genetics
Organism: Human
Tissue: Cervix
Model: Reporter
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FBS, 5% CO2, 37?‚°C. Antibiotic selection for GFP and mCherry expression: 1µg/mL Puromycin, 4µg/mL Blasticidine, expression is quite stable but selecting at least every two passages is recommended.
Recommended controls: HeLa parental line
References:

Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.

Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.

Plk1 negatively regulates Cep55 recruitment …

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