HeLa GRASP55 knockout cell line

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology was used to knock out Golgi reassembly stacking protein of 65 kDa (GRASP65) and/or Golgi reassembly stacking protein of 55 kDa (GRASP55) in HeLa and HEK293 cells. By knocking out GRASPs it was demonstrated that these proteins are essential for Golgi stacking structure formation and accurate posttranslational […]

Contributors

Inventor Institute
Yanzhuang Wang University of Michigan
Cat. #: 157706
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology
Organism: Human
Tissue: Cervix
Model: Knock-Out
Product description: Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology was used to knock out Golgi reassembly stacking protein of 65 kDa (GRASP65) and/or Golgi reassembly stacking protein of 55 kDa (GRASP55) in HeLa and HEK293 cells. By knocking out GRASPs it was demonstrated that these proteins are essential for Golgi stacking structure formation and accurate posttranslational modifications function in the Golgi. The GRASP knock out cell lines can be used to study the role of GRASPs in Golgi-related biological processes and diseases in which the Golgi is defective (e.g. Alzheimers disease, congenital disorders of glycosylation, foot-and-mouth disease, reoxygenation injury and cancer). The HeLa WT cell line can be provided, as a positive control, if requested with order.CRISPR edited HeLa cells.
Production details: Duplexed sgRNA oligos were digested and ligated into pSpCas9(BB)-2A-GFP(PX458) and pSpCas9(BB)-2A-Puro(PX459)v plasmids to generate GRASP55/65 GFP or Puro plasmids, respectively. CRISPR knock out cells were generated by transfection with GRASP55/65 GFP or Puro plasmids followed by enrichment of GFP-expressing cells by FACS sorting or by selection with 1 Â?„?žg/ml puromycin, respectively. Individual clones were generated by plating cells at low density and isolating individual colonies. GRA…
Additional notes: The HeLa WT cell line can be provided, as a positive control, if requested with order. CRISPR edited HeLa cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop an…
Parental cell line: HeLa cell line
Format: Frozen
Shipping conditions: Dry ice
Recommended controls: HeLa parental line
References:

Bekier ME et al. 2017. Mol Biol Cell. 28(21):2833-2842. PMID: 28814501.

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