#160839

HeLa Flp-In SLC7A5

Cat. #160839

HeLa Flp-In SLC7A5

Cat. #: 160839

Unit size: 1x10^6 cells / vial

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Matthias Hentze

Institute: European Molecular Biology Laboratory (EMBL)

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: HeLa Flp-In SLC7A5
  • Research fields: Metabolism
  • Parental cell: HeLa FLP-IN T-REx
  • Model: Knock-Out
  • Description: To identify genes whose expression is controlled by YBX3
  • Application: YBX3
  • Production details: Adherent HeLa cells were grown at 37Â?‚°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO). Cells were grown until 70%â?‚€?‚“80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerâ?‚€?‚™s recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from...

Target Details

  • Target: YBX3

Applications

  • Application: YBX3

Handling

  • Format: Frozen
  • Growth medium: Adherent HeLa cells were grown at 37?‚°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO) Grown in medium containing blasticidine (5mg/ml) Cells were grown until 70%?‚–80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturer?‚Â’s recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and con...
  • Unit size: 1x10^6 cells / vial

Related Tools

  • Related tools: HeLa Flp-In YBX3: GFP ; HeLa Flp-In SLC7A5+SLC7A5 3â?‚€?‹Â› UTR ; HeLa Flp-In SLC25A6 ; HeLa Flp-In SLC25A6+SLC7A5 3â?‚€?‹Â› UTR

References

  • The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance