HeLa/EGFP_Cytochrome c

Cervical cancer cell line HeLa stably expressing Cytochrome c-EGFP for use as an apoptosis sensor.

Contributors

Inventor Institute
T.R. Santhosh Kumar Rajiv Gandhi Centre for Biotechnology
Cat. #: 161151
Cancer types: Gynaecologic cancer
Research Fields: Cancer;Disease (not cancer);Metabolism
Organism: Human
Tissue: Cervix
Gender: Female
Model: Transgenic
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Cervical
Primary citation: Srinivas KP, et.al. PMID:26992219
Product description: The product is a human cervical carcinoma cell line HeLa, transfected to stably express the protein cytochrome c (cyt-c) tagged with EGFP. The EGFP-tag enables easy identification of the cyt-c release in live cells upon apoptotic induction. The cell line can be utilized for studying the apoptosis process and to screen of potential candidate drugs that induce apoptosis.Stably expressing single cell clones with homogeneous expression of Cytochrome c_EGFP were isolated for high resolution imaging. The expression and functionality of exogenous Cytochrome c_EGFP was confirmed through fluorescence microscopy by its release upon apoptotic stimulus.
Gender: Female
CRISPR: No
Conditional: No
Parental cell line: HeLa
disease: Cancer
Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or EMEM with 2mM L-Glutamine and 10% Fetal Bovine Serum.
Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: Growth medium + 10% DMSO
References:

Srinivas KP, et.al. PMID:26992219

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