HEK 293S Cell Line

The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA.

Contributors

Institute
Vlaams Instituut voor Biotechnologie (VIB)
Cat. #: 154155
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: Kidney
Model: Immortalised Line
Product description: The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA. The human embryonic kidney cells at first seemed recalcitrant to transformation. After many attempts, cell growth took off only several months after the isolation of a single transformed clone. A 4-kbp adenoviral genome fragment is known to have integrated in chromosome 19 and encodes for the E1A/E1B proteins, which interfere with the cell cycle control pathways and counteract apoptosis. Cytogenetic analysis established that the 293 line is pseudotriploid. HEK 293S cell line is adapted for culture in suspension. These HEK 293S have been whole-genome re-sequenced at early passage. The article by Lin et al al, allows user friendly visualisation of the genome data. The genome structure composition is in steady state when standard cell culturing conditions are used.
Conditional: No
Production details: Hek 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA
Cellosaurus ID: CVCL_A784
Parental cell line: Human embryonic kidney cells
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: EMEM (EBSS) + 2mM Glutamine + 1% Non-Essential Amino Acids (NEAA) + 10% FCS
Mycoplasma free: Yes
Biosafety level: 1
References:

Lin et al. 2014. Nat Commun. 5:4767. PMID: 25182477.

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.

Stillman et al. 1985. Mol Cell Biol. 5(8):2051-60. PMID: 3018548.

Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.

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