GCGR E55 Cell line

The GCGR cell lines comprise a panel of primary, patient‑derived IDH‑wildtype glioblastoma stem‑like models developed to capture inter‑patient heterogeneity in tumour growth, behaviour, and molecular features.

Contributors

Inventor Institute
Gillian Morrison, Steven Pollard University of Edinburgh
Cat. #: 140079
Tool sub type: Primary
Organism: Human
Tissue: Glioblastoma
Model: Primary cell line
Morphology: Adherent monolayer with stem-like features (SOX2, NESTIN).
Growth properties: Adherent
Cancer Types In Detail: Glioblastoma
Product description: GCGR‑E55 is a primary, patient‑derived, IDH‑wildtype glioblastoma stem‑like cell line derived from human tumour tissue. The line grows as an adherent, laminin‑dependent monolayer, has a doubling time of approximately 65 hours, and forms tumours in vivo. Early‑passage transcriptomic profiling (P3) indicated a mesenchymal‑like transcriptional state based on probability scoring. The line shows an injury‑associated gene expression phenotype with enrichment for mesenchymal‑immune (MESImm) gene programmes. GCGR‑E55 is applied in studies of glioblastoma stem‑like cell behaviour, tumourigenicity, and pathways associated with mesenchymal‑like states, supporting viability and phenotypic assays.
Production details: Derived from surgically resected primary glioblastoma tissue and expanded as an adherent monolayer under laminin?supported condition
Additional notes: This cell line is a primary glioblastoma stem‑like model derived from a single patient tumour and exhibits intrinsic cellular heterogeneity, reflecting the diversity and plasticity typical of glioblastoma. Transcriptional state classifications were assessed at early passage (P3) and may change in culture
Format: Frozen
Growth medium: Serum‑free DMEM/HAMS‑F12 (“Complete + EFL”) supplemented with glucose, NEAA, B27, N2, BSA, β‑mercaptoethanol, Pen/Strep, and recombinant EGF (10 ng/mL), FGF‑2 (10 ng/mL), plus laminin‑1 (2 µg/mL). Laminin is required for adherent growth.
Subculture routine: Passage at 80–90% confluence; split 1:4–1:6; detach using Accutase; avoid over‑dilution; replace medium weekly
Recommended controls: GCGR human neural stem cell lines
Biosafety level: 2
References:

Pollard SM et al. Cell Stem Cell 2009 PMID: 19497285.

Furqan M et al. Pharmaceutics 2025 PMID: 40430842.

Related Tools for Cell lines

Cat. # Tool Name
151449 JIM1 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151480 HEK293A EGFP-LC3 Cell Line Key Info View Tool
151576 MEF RAF1 KO Cell Line Key Info View Tool
151630 PDVC57B Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.