The pOG44 Flp-recombinase expression vector was co-transfected with the EX2-antisense (AS) reporter construct into Flp-InÄË?Â? T-RExÄË?Â? 293 cells
Parental cell line:
Flp-In T-REx 293 cell line
Format:
Frozen
Shipping conditions:
Dry ice
Growth medium:
maintain under selection with 200 ?g/ml Hygromycin B and 15 ?g/ml Blasticidin at 37?°C in a humidified atmosphere with 5% CO2.
Recommended controls:
Flp-In? T-REx? 293 cell line
References:
Vtor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.
(A) Schematics of the EX2-AS reporter constructed to directly inspect break-induced antisense transcription initiation. (B) Representative images of a total of eight imaged cells and dual-line plot of a recorded cell where break-induced antisense transcription initiation was detected. (Sci Adv. 2019 Jan 9;5(1)
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