#162180

ENO 1 F423A M1 HAP1 cell line

Cat. #162180

ENO 1 F423A M1 HAP1 cell line

Cat. #: 162180

Organism: Human

Tissue: Bone marrow

Disease: Membranous glomerulonephritis

Model: Genetically modified cell line; knock-in

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Marit Otterlei

Institute: Norwegian University of Science and Technology

Tool Details
Target Details
Applications
Handling
Related Tools

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: ENO 1 F423A M1 HAP1 cell line
  • Parental cell: HAP1
  • Organism: Human
  • Gender: Male
  • Tissue: Bone marrow
  • Donor: 40 years old
  • Disease: Membranous glomerulonephritis
  • Morphology: Fibroblast-like
  • Growth properties: Adherent
  • Model: Genetically modified cell line; knock-in
  • Model description: mutated PCNA interacting domain APIM (F423A) in the ENO1 gene
  • Crispr: Yes
  • Products or characteristics of interest: near-haploid cell line containing half of the usual number of chromosomes
  • Description: Chronic myelogenous leukaemia cell line with mutated PCNA interacting domain APIM (F423A) in the ?-Enolase (ENO1) glycolytic enzyme (clone M1)
  • Application: Cell culture, immunofluorescence, metabolic function assays

Target Details

  • Target: PCNA interacting domain APIM in the ENO1 gene

Applications

  • Application: Cell culture, immunofluorescence, metabolic function assays

Handling

  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Subculture routine: Cells should be kept at maximum 75% confluency
  • Characterisation tests: 6-phospho gluconate dehydrogenase assay, Western Blot, immunofluorescence

Related Tools

  • Related tools: ENO 1 F423A M2 HAP1 cell line