Cat. #162158
Dnmt-TKO cell line
Cat. #: 162158
Sub-type: Primary
Organism: Mouse
Tissue: Blastocyst
Model: Genetically modified cell line; Knock out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Institut Curie
Primary Citation: Mathieu Schulz et. al. 2024 Nat Struct Mol Biol. 31(1):102-114. PMID: 38177678
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Dnmt-TKO cell line
- Research fields: Stem cell biology
- Tool sub type: Primary
- Parental cell: E14 murine ES cells (129Ola strain)
- Organism: Mouse
- Gender: Male
- Tissue: Blastocyst
- Growth properties: Adherent
- Model: Genetically modified cell line; Knock out
- Crispr: Yes
- Description: Dnmt triple KO line from low-passage ESCs (E14 background) showing allelic homozygous deletions of Dnmt genes after catalytic motif targeting by CRISPRCas9. This line is constitutively free from DNA methylation.
Handling
- Format: Frozen
- Passage number: P5
- Growth medium: Choice of media depends on the aim of the experiment - please see primary citation for details. Potential ESC media include: (1) serum + LIF (2) 2i + LIF to stabilise pluripotency (3) 2i + vitamin C, to reduce DNA methylation levels
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Cultured in antibiotics: Yes
- Characterisation tests: Western blotting to confirm the depletion of the protein
References
- Mathieu Schulz et. al. 2024 Nat Struct Mol Biol. 31(1):102-114. PMID: 38177678
