Dnmt-TKO cell line

Murine E14 embyonic line constitutively free from DNA methylation

Contributors

Institute
Institut Curie
Cat. #: 162158
Tool sub type: Primary
Research Fields: Stem cell biology
Organism: Mouse
Tissue: Blastocyst
Gender: Male
Model: Genetically modified cell line; Knock out
Growth properties: Adherent
Primary citation: Mathieu Schulz et. al. 2024 Nat Struct Mol Biol. 31(1):102-114. PMID: 38177678
Product description: Dnmt triple KO line from low-passage ESCs (E14 background) showing allelic homozygous deletions of Dnmt genes after catalytic motif targeting by CRISPRCas9. This line is constitutively free from DNA methylation.
Gender: Male
CRISPR: Yes
Parental cell line: E14 murine ES cells (129Ola strain)
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Choice of media depends on the aim of the experiment – please see primary citation for details. Potential ESC media include: (1) serum + LIF (2) 2i + LIF to stabilise pluripotency (3) 2i + vitamin C, to reduce DNA methylation levels
Temperature: 37° C
Atmosphere: 5% CO2
References:

Mathieu Schulz et. al. 2024 Nat Struct Mol Biol. 31(1):102-114. PMID: 38177678

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