DLKP-pHA MDR 1/A cell line

DLKP-pHA MDR 1/A cell line: heterogeneous lung carcinoma population. Epithelial model

Contributors

Inventor Institute
Martin Clynes Dublin City University
Cat. #: 157981
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Lung cancer
Research Fields: Cancer;Cell biology;Drug development
Organism: Human
Tissue: Lymph Node
Growth properties: monolayer, slow-growing
Cancer Types In Detail: Lung carcinoma
Product description: heterogeneous lung carcinoma population. Epithelial model
Conditional description: fold resistance comp. toÂ?‚ DLKP parent: 15-fold Adriamycin; 12.2 Vincristin; 13.6 VP-16 (Etoposide).
Production details: DLKPÂ?‚ transfected with a pHaMDR1/A plasmid (full cDNA for human MDR1 gene) and treated with 50mg/ml adriamycin
Parental cell line: DLKP
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM/Ham's F12, 5% FCS supplement with 50ng/ml adriamycin at least once/week. Passage 2-3 time weekly, spin after trypsinisation. Freezing: 5% DMSO, freeze at 2×10(6) cells/ml, spin down after reviving
Recommended controls: DLKP parental line
Mycoplasma free: Yes
Biosafety level: 1

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