| Inventor | Institute |
|---|---|
| Jorgen Fogh, James D. Loveless | Memorial Sloan-Kettering Cancer Center (MSK) |
| SKU: | 161908 |
|---|---|
| Product description: | Capan-2 is a human pancreatic ductal adenocarcinoma cell line. These cells grow in adherent tissue culture and display epithelial morphology. They form well-differentiated tumours when injected into immunocompromised mice and are used as a xenograft model for pancreatic cancer. The Capan-2 cells express mutant K-Ras (G12V) and elevated levels of the Epidermal Growth Factor Receptor (EGFR). In addition, they express wildtype p53 and normal levels of SMAD4 protein. |
| Gender: | Male |
| Cellosaurus ID: | CVCL_0026 |
| Products or characteristics of interest: | Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1-2 |
| Cat. #: | 161908 |
|---|---|
| Organism: | Human |
| Tissue: | Pancreas |
| Gender: | Male |
| Morphology: | Polygonal |
| Growth properties: | Adherent |
| Primary citation: | Kyriazis et al. 1986. Cancer Research. 46: 5810-5815. PMID: 3019537. |
| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | McCoy’s 5a Medium Modified supplemented with 10% FBS |
| Subculture routine: | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Temperature: | 37° C |
| Atmosphere: | 95% Air, 5% CO2 |
| Storage medium: | Culture medium 95%; DMSO, 5% |
| Biosafety level: | 1 |
| References: |
Kyriazis et al. 1986. Cancer Research. 46: 5810-5815. PMID: 3019537. Deer et al. 2010. Pancreas. 39: 425-435. PMID: 20418756. |
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