Capan-2 Cell line

Capan-2 is a human pancreatic ductal adenocarcinoma cell line. These cells grow in adherent tissue culture and display epithelial morphology. They form well-differentiated tumours when injected into immunocompromised mice and are used as a xenograft model for pancreatic cancer. The Capan-2 cells express mutant K-Ras (G12V) and elevated levels of the Epidermal Growth Factor Receptor […]

Contributors

Inventor Institute
Jorgen Fogh, James D. Loveless Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161908
Organism: Human
Tissue: Pancreas
Gender: Male
Morphology: Polygonal
Growth properties: Adherent
Primary citation: Kyriazis et al. 1986. Cancer Research. 46: 5810-5815. PMID: 3019537.
Product description: Capan-2 is a human pancreatic ductal adenocarcinoma cell line. These cells grow in adherent tissue culture and display epithelial morphology. They form well-differentiated tumours when injected into immunocompromised mice and are used as a xenograft model for pancreatic cancer. The Capan-2 cells express mutant K-Ras (G12V) and elevated levels of the Epidermal Growth Factor Receptor (EGFR). In addition, they express wildtype p53 and normal levels of SMAD4 protein.
Gender: Male
Products or characteristics of interest: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1-2
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: McCoy's 5a Medium Modified supplemented with 10% FBS
Subculture routine: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Temperature: 37° C
Atmosphere: 95% Air, 5% CO2
Storage medium: Culture medium 95%; DMSO, 5%
Biosafety level: 1
References:

Kyriazis et al. 1986. Cancer Research. 46: 5810-5815. PMID: 3019537.

Deer et al. 2010. Pancreas. 39: 425-435. PMID: 20418756.

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