786O PBRM1 KO2

786O PBRM1 KO2

Availability: 3-4 weeks

Organism: Human

Tissue: kidney

Model: Genetically modified


This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.


Inventor: Jessica Downs, Karen Lane

Institute: The Institute of Cancer Research

Tool Details

Tool Details


  • Name: 786O PBRM1 KO2
  • Parental cell: 786-O
  • Clone: 2 (also called clone 2.7)
  • Organism: Human
  • Gender: Male
  • Tissue: kidney
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Genetically modified
  • Model description: PBRM1 knockout
  • Crispr: Yes
  • Conditional: No
  • Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
  • Description: 786-O cell line knockout for PBRM1 (clone 2, also called clone 2.7). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
  • Application: Chromatin remodelling biology studies
  • Biosafety level: 1
  • Cellosaurus id: CVCL_1051


  • Application: Chromatin remodelling biology studies


  • Format: Frozen
  • Growth medium: RPMI-1640+ 10%FBS + 1% Penicillin/Streptomycin
  • Temperature: 37C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 1% Penicillin/Streptomycin
  • Mycoplasma free: Yes
  • Str profiling: See Cellosaurus profile of parental cell line


  • Unpublished