Cat. #153249
3.334 SC5/8 Cell Line
Cat. #: 153249
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Hybrid
Tissue: Fibroblast
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Chris Marshall
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 3.334 SC5/8 Cell Line
- Research fields: Cell biology
- Tool sub type: Continuous
- Parental cell: 3T3TK3 cells and normal rat kidney cells
- Organism: Hybrid
- Tissue: Fibroblast
- Growth properties: Adherent
- Model: Tumour line
- Conditional: No
- Description: Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
- Additional notes: Rat x Mouse hybrid
- Cellosaurus id: CVCL_7237
Applications
- Application notes: Rat x Mouse hybrid
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm? using 0.25% trypsin/EDTA; 35?°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.
- Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.
