#153249

3.334 SC5/8 Cell Line

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Cat. #153249

3.334 SC5/8 Cell Line

Cat. #: 153249

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Hybrid

Tissue: Fibroblast

Model: Tumour line

£575.00

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Contributor

Inventor: Chris Marshall

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: 3.334 SC5/8 Cell Line
  • Research fields: Cell biology
  • Tool sub type: Continuous
  • Parental cell: 3T3TK3 cells and normal rat kidney cells
  • Organism: Hybrid
  • Tissue: Fibroblast
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: No
  • Description: Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
  • Additional notes: Rat x Mouse hybrid
  • Cellosaurus id: CVCL_7237

Applications

  • Application notes: Rat x Mouse hybrid

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm?‹Â› using 0.25% trypsin/EDTA; 35?‚°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.
  • Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.