#161801

2XSB Cell Line

Cat. #161801

2XSB Cell Line

Cat. #: 161801

Organism: Human

Tissue: Neoplasm from right brachial nerve (WHO grade IV sporadic MPNST)

£575.00

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Contributor

Inventor: Steven Carroll

Institute: The UAB Research Foundation

Primary Citation: Longo et. al. 2021. Sci Rep 11, 5690. PMID: 33707600

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: 2XSB Cell Line
  • Alternate name: 2XSB Cell Line
  • Organism: Human
  • Gender: Female
  • Tissue: Neoplasm from right brachial nerve (WHO grade IV sporadic MPNST)
  • Donor: 57 year old Caucasian woman with no previous history of cancer
  • Morphology: Spindled to polygonal morphology with multiple cytoplasmic vacuoles. Subpopulation of cells that were larger and multinucleated also present.
  • Growth properties: Adherent
  • Crispr: No
  • Receptors of note: No
  • Description: Cell line derived from human with malignant peripheral nerve sheath tumors (MPNSTs). Cell line shares molecular and genomic features of parent tumor and is able to form solid tumors when xenografted into immunodeficient mice. 2XSB cells have functional NF1 alleles and no mutations in genes encoding the Polycomb Repressor Complex 2. Mutations in TP53 and PTEN were detected as well as a homozygous deletion of CDKN2A which aregenes implicated in MPNST pathogenesis. Other mutations included DNMT1...
  • Application: Karyotyping, STR profiling, ICC, IHC, Live cell imaging, TMEM, SNP, whole exome sequencing, TERT promoter Sanger seq, Western blot, and Ras activation assays.
  • Production details: Established from fresh tumor tissue that was placed in clulture plates with DMEM supplementeed with fetal calf serum, Schwann cell mitogen, forskolin, glutamin, streptomycin, and penicillin. Tissue was minced and allowed to migrate out of tissue for 72 hours. Afterwards, non-dispersed tissue was rinsed with PBS and trypsinized. DMEM and fetal calf serum was added and cells were centrifuged, resuspended, and added to cells that had previously migrated. Culture was llowed to grow until confluen...

Applications

  • Application: Karyotyping, STR profiling, ICC, IHC, Live cell imaging, TMEM, SNP, whole exome sequencing, TERT promoter Sanger seq, Western blot, and Ras activation assays.

Handling

  • Growth medium: DMEM, 10% fetal calf serum, 10nM NRG1B, 2uM forskolin, 1% glutamine, 10ug/mL streptomycin, and 10IU/mL penicillin
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Cultured in antibiotics: Streptomycin and Penicillin

References

  • Longo et. al. 2021. Sci Rep 11, 5690. PMID: 33707600