#153752

16E6-E7 HFK

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Cat. #153752

16E6-E7 HFK

Cat. #: 153752

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Disease: Cancer

Model: Immortalised Line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Aloysius Klingelhutz

Institute: The University of Iowa

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: 16E6-E7 HFK
  • Research fields: Cancer
  • Tool sub type: Continuous
  • Parental cell: Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
  • Organism: Human
  • Disease: Cancer
  • Model: Immortalised Line
  • Conditional: No
  • Description: Expresses skin cell markers; proinflammatory. Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
  • Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
  • Production details: HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance.Â?‚ 
  • Biosafety level: 1

Target Details

  • Target: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies

Applications

  • Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies

Handling

  • Format: Frozen
  • Growth medium: Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.