143B m.8993T>G isogenic mTUNE mT70 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: 143B m.8993T>G isogenic mTUNE mT70 Cell Line
• Alternate name: mTUNE 7, mT7
• Tool sub type: Continuous
• Parental cell: 143B human osteosarcoma (m.8993T>G cybrid)
• Organism: Human
• Tissue: Bone
• Disease: Cancer
• Model: Tumour line
• Description: The cellular metabolic environemnt is influenced by relative levels of coexistant mutant and wild-type mitochondrial DNAs (heteorplasmy) in a range of diseases for which mitochondrial dysfunction is a feature. Disease phenotypes may be rescued by returning heteroplasmy towards wild-type levels. To investigate this approach, the team led by Payam Gammage have devised a series of cell lines which stably express stable levels of mutatnt m.8993T>G mtDNA heteroplasmy. Cell lines bearing m.8993T>G heteroplasmy of 70 and 10% are available at CancerTools.org. These lines are derivatives of the m.8993T cybrid cell developed by Prof Eric Schon, and are of of 143B human osteosarcoma lineage. This is part of a series of two cell line (mTUNE); see Related research tools tab.
• Production details: mTUNE cells were generated by Dr Michal Minczukâ?‚€?‚™s lab and derive from female human osteosarcoma 143B (RRID: CVCL_2270) cybrid cells (Porteous et al., 1998), after correction of m.8993T>G mutation with mitochondrially-targeted zinc finger nucleases (Gammage et al., 2016a). See Gaude et al., 2018 for further details.
• Additional notes: This is part of a series of two cell line (mTUNE); see Related Reagents tab.

Handling

• Format: Frozen
• Growth medium: high glucose DMEM media supplemented with L-glutamine and 10S and pen/strep
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Subculture routine: Grow in regular dishes or flasks (10cm dish or 75cm2 flask). Passage 2 or 3 times a week, split when confluent (wash with PBS and then use trypsin to split). There is no maximum passage number. 1:10 for 3 splits a week, 1:20 Friday to Monday

Related Tools

• Related tools: 143B m.8993T>G isogenic mTUNE mT10 Cell Line

References

• Genome editing in mitochondria corrects a pathogenic mtDNA mutation in vivo.
• Enhanced Manipulation of Human Mitochondrial DNA Heteroplasmy In Vitro Using Tunable mtZFN Technology.
• NADH Shuttling Couples Cytosolic Reductive Carboxylation of Glutamine with Glycolysis in Cells with Mitochondrial Dysfunction.
• Engineered mtZFNs for Manipulation of Human Mitochondrial DNA Heteroplasmy.
• Near-complete elimination of mutant mtDNA by iterative or dynamic dose-controlled treatment with mtZFNs....