Cat. #151515
Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]
Cat. #: 151515
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Zebrafish Basolateral Pole of Cells
Class: Monoclonal
Application: IHC ; IHC ; IF ; WB
Reactivity: Zebrafish
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Linda Ariza-McNaughton
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]
- Research fields: Cell biology;Developmental biology;Tags and cell markers
- Clone: FIS 2H9/1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 170 kDa
- Strain: Balb/c
- Reactivity: Zebrafish
- Host: Mouse
- Application: IHC ; IHC ; IF ; WB
- Description: This antibody is a useful marker of apicobasal polarity in the epithelium.
- Immunogen: Lysate of zebrafish intestine
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Zebrafish Basolateral Pole of Cells
- Molecular weight: 170 kDa
- Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 2H9 recognises a membrane-associated epitope on the gut epithelial cells, concentrated basolaterally. In one month old zebrafish it appears especially prominent in the basal parts of the elongated cells of the villi.
Applications
- Application: IHC ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
Related Tools
- Related tools: Anti-Zebrafish gut secretory cell epitopes [FIS 6G5/1] ; Anti-Zebrafish gut secretory cell epitopes [FIS 4B7/2] ; Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1] ; Anti-Zebrafish basement membrane marker [FIS 5F11/2] ; Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1] ; Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1] ; Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2] ; Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
References
- Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
- Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.
