#151414

Anti-Ufd1 [5e2]

Cat. #151414

Anti-Ufd1 [5e2]

Cat. #: 151414

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Ufd1

Class: Monoclonal

Application: ELISA ; IF ; IP ; WB

Reactivity: Human ; Rat ; Mouse ; Xenopus laevis

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-Ufd1 [5e2]
  • Research fields: Cancer;Cell biology
  • Clone: 500
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 40 kDa
  • Strain: Balb/c
  • Reactivity: Human ; Rat ; Mouse ; Xenopus laevis
  • Host: Mouse
  • Application: ELISA ; IF ; IP ; WB
  • Description: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.
  • Immunogen: Full length mouse Ufd1 expressed in E. coli
  • Isotype: IgG2a
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: HeLa or HEK293 cell lysate.

Target Details

  • Target: Ufd1
  • Molecular weight: 40 kDa
  • Tissue cell line specificity: HeLa or HEK293 cell lysate.
  • Target background: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.

Applications

  • Application: ELISA ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.
  • The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
  • Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.
  • Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
  • Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.
  • A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.