Anti-Ufd1 [5e2]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Ufd1 [5e2]
• Research fields: Cancer;Cell biology
• Clone: 500
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 40 kDa
• Strain: Balb/c
• Reactivity: Human ; Rat ; Mouse ; Xenopus laevis
• Host: Mouse
• Application: ELISA ; IF ; IP ; WB
• Description: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.
• Immunogen: Full length mouse Ufd1 expressed in E. coli
• Isotype: IgG2a
• Myeloma used: Sp2/0-Ag14
• Recommended controls: HeLa or HEK293 cell lysate.

Target Details

• Target: Ufd1
• Molecular weight: 40 kDa
• Tissue cell line specificity: HeLa or HEK293 cell lysate.
• Target background: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.

Applications

• Application: ELISA ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.
• The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
• Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.
• Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
• Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.
• A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.