Cat. #151196
Anti-Rad51 [Rad51 3C10] mAb
Cat. #: 151196
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Rad51
Class: Monoclonal
Application: IHC ; IP ; WB
Reactivity: Bovine ; Human ; Pig
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stephen West
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Rad51 [Rad51 3C10] mAb
- Research fields: Genetics
- Clone: Rad51 3C10
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Bovine ; Human ; Pig
- Host: Mouse
- Application: IHC ; IP ; WB
- Description: Monoclonal antibody which binds RAD51 recombinase protein, associated with DNA repair pathway.
- Immunogen: Full length recombinant human RAD51 protein
- Immunogen uniprot id: Q06609
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Rad51
- Target background: RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51 catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Mutations in the gene can results in breast cancer, mirror movements 2 and Fanconi anaemia.
Applications
- Application: IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-Rad51 [Rad51 3C10] recombinant antibody
References
- Vohhodina et al. 2017. Nucleic Acids Res. 45(22):12816-12833. PMID: 29112714.
- Subramanyam et al. 2016. Proc Natl Acad Sci U S A. 113(41):E6045-E6054. PMID: 27671650.
- Marampon et al. 2016. Oncotarget. 7(5):5383-400. PMID: 26689991.
- Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.
- Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.
- Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.
- The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
- Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.
- Distribution of the Rad51 recombinase in human and mouse spermatocytes.
