Cat. #153350
Anti-Plakoglobin [VB3]
Cat. #: 153350
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: Plakoglobin
Class: Polyclonal
Application: IP ; IF ; IP ; WB
Reactivity: Human ; Mouse
Host: Rabbit
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Fiona Watt
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Plakoglobin [VB3]
- Alternate name: Gamma Catenin
- Research fields: Cell biology
- Clone: VB3
- Tool sub type: Primary antibody
- Class: Polyclonal
- Conjugation: Unconjugated
- Reactivity: Human ; Mouse
- Host: Rabbit
- Application: IP ; IF ; IP ; WB
- Description: Plakoglobin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
- Immunogen: Peptide - NH2-CIDTYSDGLRPPYPTADH-COOH
- Recommended controls: A431 or HaCaT cell lysate
Target Details
- Target: Plakoglobin
- Tissue cell line specificity: A431 or HaCaT cell lysate
- Target background: Plakoglobin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Applications
- Application: IP ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: Serum
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Erasmus et al. 2016. Nat Commun. 7:13542. PMID: 27922008.
- Defining Fn interactions during biogenesis of epithelial junctions.
- Ryan et al. 2012. J Cell Sci. 125(Pt 13):3202-9. PMID: 22454524.
- Plakoglobin-dependent regulation of keratinocyte apoptosis by Rnd3.
- Zhu et al. 1999. Development. 126(10):2285-98. PMID: 10207152.
- beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion.
- Braga et al. 1995. Cell Adhes Commun. 3(3):201-15. PMID: 8846022.
- Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
- NĤthke et al. 1994. J Cell Biol. 125(6):1341-52. PMID: 8207062.
- Defining interactions and distributions of cadherin and catenin complexes in polarized epithelial cells.
