Anti-Phospho CHMP4C [pCHMP4C]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Phospho CHMP4C [pCHMP4C]
• Research fields: Cell biology
• Clone: pCHMP4C
• Class: Polyclonal
• Conjugation: Unconjugated
• Molecular weight: ~30
• Reactivity: Mammalian
• Host: Rabbit
• Application: WB
• Description: Borealin interacts directly with Snf7/Shrb/CHMP4 components in both Drosophila and human cells and the two proteins colocalize at the midbody in late cytokinesis. Aurora B phosphorylates CHMP4C at three serine residues located in its C-terminal linker region, a part of the protein known to regulate its ability to form polymers and interact with the membrane. Over-expression of CHMP4C variants mutated in these three residues caused cytokinesis failure, suggesting that Aurora B inhibits CHMP4C activity during cytokinesis. It is proposed that CPC controls abscission timing in both flies and human cells by regulating the function of ESCRT-III Snf7 proteins during cytokinesis through the interaction of its Borealin component with the N-terminus of Shrb/CHMP4 proteins and Aurora B-mediated phosphorylation of the CHMP4C regulatory linker tail.
• Immunogen: Synthetic peptide (TARRSRAASSQRAEEC)
• Recommended controls: Synchronised HeLa cell extracts. Signals are absent in a twin blot that had been pre-incubated with lambda-phosphatase, indicating that the antibody specifically recognizes a phosphorylated form of CHMP4C.

Target Details

• Target: Phospho charged multi vesicular protein 4c
• Molecular weight: ~30
• Target background: Borealin interacts directly with Snf7/Shrb/CHMP4 components in both Drosophila and human cells and the two proteins colocalize at the midbody in late cytokinesis. Aurora B phosphorylates CHMP4C at three serine residues located in its C-terminal linker region, a part of the protein known to regulate its ability to form polymers and interact with the membrane. Over-expression of CHMP4C variants mutated in these three residues caused cytokinesis failure, suggesting that Aurora B inhibits CHMP4C activity during cytokinesis. It is proposed that CPC controls abscission timing in both flies and human cells by regulating the function of ESCRT-III Snf7 proteins during cytokinesis through the interaction of its Borealin component with the N-terminus of Shrb/CHMP4 proteins and Aurora B-mediated phosphorylation of the CHMP4C regulatory linker tail.

Applications

• Application: WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -20° C
• Shipping conditions: Shipping at 4° C

References

• Capalbo et al. 2012. Open Biol. 2:120070 PMID: 22724069
• Petsalaki et al. 2016. Nat Commun. 7:11451. PMID: 27126587.