Cat. #151146
Anti-p53 [Pab 240]
Cat. #: 151146
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: p53
Class: Monoclonal
Application: FACS ; IHC ; IF ; IP ; WB
Reactivity: Chicken ; Mammalian
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: David Lane
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Primary Citation: Greaves. R. F (1988) Ph.D Thesis. University of London.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-p53 [Pab 240]
- Alternate name: Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; P53 Tumor Suppressor; Tumor Suppressor P53; TRP53; BCC7; LFS1
- Cancers detailed: Broadly Applicable
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
- Clone: Pab 240
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 53 kDa
- Strain: Balb/c
- Reactivity: Chicken ; Mammalian
- Host: Mouse
- Disease: Breast Cancer
- Application: FACS ; IHC ; IF ; IP ; WB
- Description: This monoclonal antibody reacts specifically with mutated p53 at an epitope conserved across species and normally hidden within the protein structure in non-mutant forms. Anti-p53 Pab 240 was created further understand the biochemical role of p53 in cancers and the precise effects of mutation. Anti-p53 (Pab 240) antibody recognises an evolutionarily conserved epitope upon p53 hidden within the normal protein structure, however, it does not bind to immunoprecipitated wild-type p53. This antibo...
- Immunogen: p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)
- Immunogen uniprot id: P04637
- Isotype: IgG1 kappa
- Myeloma used: Sp2/0-Ag14
- Recommended controls: MDA-MB-231 cell line
Target Details
- Target: p53
- Molecular weight: 53 kDa
- Tissue cell line specificity: MDA-MB-231 cell line
- Target background: p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is the most common genetic mutational event so far identified in human cancers, with the gene present on the short arm of chromosome 17 a frequent site of allele loss in common cancers. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory doma...
Applications
- Application: FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Renzi et al. 2019. PLoS One. 14(4):e0215621. PMID: 30998743.
- Diaz Osterman et al. 2019. Elife. 8:. PMID: 31478830.
- Fekry et al. 2018. Nat Commun. 9(1):4149. PMID: 30297838.
- Katz et al. 2018. Genes Dev. 32(5-6):430-447. PMID: 29549180.
- Chen et al. 2017. Br J Pharmacol. 174(23):4345-4361. PMID: 28910492.
- Ilhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.
- Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.
- Pan et al. 2009. EMBO J. 28(22)...
