#151146

Anti-p53 [Pab 240]

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Cat. #151146

Anti-p53 [Pab 240]

Cat. #: 151146

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: p53

Class: Monoclonal

Application: FACS ; IHC ; IF ; IP ; WB

Reactivity: Chicken ; Mammalian

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: David Lane

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Primary Citation: Greaves. R. F (1988) Ph.D Thesis. University of London.

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-p53 [Pab 240]
  • Alternate name: Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; P53 Tumor Suppressor; Tumor Suppressor P53; TRP53; BCC7; LFS1
  • Cancers detailed: Broadly Applicable
  • Research fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
  • Clone: Pab 240
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 53 kDa
  • Strain: Balb/c
  • Reactivity: Chicken ; Mammalian
  • Host: Mouse
  • Disease: Breast Cancer
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: This monoclonal antibody reacts specifically with mutated p53 at an epitope conserved across species and normally hidden within the protein structure in non-mutant forms. Anti-p53 Pab 240 was created further understand the biochemical role of p53 in cancers and the precise effects of mutation. Anti-p53 (Pab 240) antibody recognises an evolutionarily conserved epitope upon p53 hidden within the normal protein structure, however, it does not bind to immunoprecipitated wild-type p53. This antibo...
  • Immunogen: p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)
  • Immunogen uniprot id: P04637
  • Isotype: IgG1 kappa
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: MDA-MB-231 cell line

Target Details

  • Target: p53
  • Molecular weight: 53 kDa
  • Tissue cell line specificity: MDA-MB-231 cell line
  • Target background: p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is the most common genetic mutational event so far identified in human cancers, with the gene present on the short arm of chromosome 17 a frequent site of allele loss in common cancers. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory doma...

Applications

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Renzi et al. 2019. PLoS One. 14(4):e0215621. PMID: 30998743.
  • Diaz Osterman et al. 2019. Elife. 8:. PMID: 31478830.
  • Fekry et al. 2018. Nat Commun. 9(1):4149. PMID: 30297838.
  • Katz et al. 2018. Genes Dev. 32(5-6):430-447. PMID: 29549180.
  • Chen et al. 2017. Br J Pharmacol. 174(23):4345-4361. PMID: 28910492.
  • Ilhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.
  • Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.
  • Pan et al. 2009. EMBO J. 28(22)...