Anti-p53 [Pab 240]

Monoclonal antibody reacts specifically with mutated p53 at an epitope conserved across species and normally hidden within the protein structure in non-mutant forms._x000D_ Anti-p53 Pab 240 was created further understand the biochemical role of p53 in cancers and the precise effects of mutation.Anti-p53 (Pab 240) antibody recognises an evolutionary conserved epitope upon p53 hidden within the normal protein structure, however it does not bind to immunoprecipitated wild type p53. This antibody recognises an epitope which is structurally elusive when p53 is in a wildtype conformation but becomes accessible upon denaturation or through mutational conformations where point mutations within the TP53…

Contributors

Inventor Institute
David Lane Cancer Research UK, London Research Institute: Clare Hall Laboratories
Cat. #: 151146
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
Application: FACS ; IHC ; IF ; IP ; WB
Target: p53
Reactivity: Chicken ; Mammalian
Clone: Pab 240
Host: Mouse
Class: Monoclonal
Primary citation: Greaves. R. F (1988) Ph.D Thesis. University of London.
Alternate name: Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; P53 Tumor Suppressor; Tumor Suppressor P53; TRP53; BCC7; LFS1
Product description: This monoclonal antibody reacts specifically with mutated p53 at an epitope conserved across species and normally hidden within the protein structure in non-mutant forms. Anti-p53 Pab 240 was created further understand the biochemical role of p53 in cancers and the precise effects of mutation. Anti-p53 (Pab 240) antibody recognises an evolutionarily conserved epitope upon p53 hidden within the normal protein structure, however, it does not bind to immunoprecipitated wild-type p53. This antibo…
Conjugation: Unconjugated
Isotype: IgG1 kappa
Molecular weight: 53 kDa
Immunogen: p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)
Immunogen Uniprot ID: P04637
Myeloma used: Sp2/0-Ag14
Target background: p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is the most common genetic mutational event so far identified in human cancers, with the gene present on the short arm of chromosome 17 a frequent site of allele loss in common cancers. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory doma…
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Renzi et al. 2019. PLoS One. 14(4):e0215621. PMID: 30998743.

Diaz Osterman et al. 2019. Elife. 8:. PMID: 31478830.

Fekry et al. 2018. Nat Commun. 9(1):4149. PMID: 30297838.

Katz et al. 2018. Genes Dev. 32(5-6):430-447. PMID: 29549180.

Chen et al. 2017. Br J Pharmacol. 174(23):4345-4361. PMID: 28910492.

Ilhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.

Pan et al. 2009. EMBO J. 28(22)…

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