Cat. #161645
Anti-NKG2D monoclonal antibody [5c6]
Cat. #: 161645
Sub-type: Primary antibody
Availability: 3-4 weeks
Target: MICA receptor NKG2D, Collagen type VI
Class: Monoclonal
Application: IP, Function Blocking
Reactivity: Human, Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Thomas Spies; Veronika Groh
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: BauerS et al. 1999. Science. 285(5428):727-9. PMID: 10426993
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-NKG2D monoclonal antibody [5c6]
- Cancer: Breast cancer;Genitourinary cancer;Gynaecologic cancer;Lung cancer
- Research fields: Immunology
- Clone: 5C6
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: 42kDa
- Strain: Can be used for: blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation.
- Reactivity: Human, Mouse
- Host: Mouse
- Application: IP, Function Blocking
- Description: Anti-human NKG2D is a monoclonal antibody to NKD2D. NKG2D is a MICA Receptor found on ?? T cells and CD8+ ?? T cells, natural killer (NK) cells and is frequently expressed in epithelial tumors. The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation
- Isotype: IgG1, ?
Target Details
- Target: MICA receptor NKG2D, Collagen type VI
- Molecular weight: 42kDa
- Target background: NKG2D is a receptor for MICA detected on most ?? T cells, CD81 ?? T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of ?? T cells and NK cells against transfectants and epithelial tumor cells expressing MICA.
Applications
- Application: IP, Function Blocking
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: The antibody solution should be stored undiluted between 2° C and 8° C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
- Shipping conditions: Dry ice
References
- BauerS et al. 1999. Science. 285(5428):727-9. DOI: 10.1126/science.285.5428.727
- V. Groh et al.1998. Science 279(1737).PMID: 9497295
