Cat. #151743
Anti-Keratin 7 & 8 [LDS68]
Cat. #: 151743
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Keratin 7, 8 in basal, prickle and intermediate cells
Class: Monoclonal
Application: IHC
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jenny Southgate
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Keratin 7 & 8 [LDS68]
- Research fields: Cell signaling and signal transduction
- Clone: LDS68
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IHC
- Description: Monoclonal antibody directed against closely related keratin's 7 and 8, useful in studying epithelial cells and tumours of epithelial origin.
- Immunogen: Cytoskeletons of the human urothelial RT4 cell line.
- Immunogen uniprot id: P08729
- Isotype: IgG1
Target Details
- Target: Keratin 7, 8 in basal, prickle and intermediate cells
- Target background: Keratins are a family of intermediate filament proteins that assemble into filaments by forming heterodimers of two different keratin strands - a type I keratin (keratins 9 to 23) and a type II keratin (keratins 1 to 8). Keratins are expressed in specific tissues, with determined differentiation patterns. They are characteristic of epithelial cells and trichocytes. Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19. The LdS68 antibody is useful for studies of epithelial cells and tumour origin. This antibody reacts specifically with a variety of human simple epithelia (e.g. bile ducts, mammary gland, sweat glands, urinary bladder, endocervix, placenta), of the stomach, colon, pancreatic acini, hepatocytes and myoepithelial cells. It does not react with epidermis or non-epithelial tissues.
Applications
- Application: IHC
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Southgate et al. 1987. Lab Invest. 56(2):211-23. PMID: 2433501.
- Primary culture of human oral epithelial cells. Growth requirements and expression of differentiated characteristics.
- Trejdosiewicz et al. 1986. Exp Cell Res. 164(2):388-98. PMID: 2423348.
- Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line.
