#161663

Anti-integrin b2

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Cat. #161663

Anti-integrin b2

Cat. #: 161663

Sub-type: Primary antibody

Target: CD18 (integrin ?2; ? subunit of CD11a, b, c)

Class: Monoclonal

Application: FACS, Function Blocking, Immunohistochemistry

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Elizabeth A. Wayner

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: Garcia-Pardo et al.1990. Journal of Immunology.144(9):3361-6. PMID: 2139453

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-integrin b2
  • Research fields: Cell biology
  • Clone: P4H9
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Molecular weight: Predicted: 85 kDa; Apparent: 93 kDa
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS, Function Blocking, Immunohistochemistry
  • Description: CD18 is a general marker of leukocytes.
  • Immunogen: Human lymphokine activated T-cells (LAK)
  • Isotype: IgG3

Target Details

  • Target: CD18 (integrin ?2; ? subunit of CD11a, b, c)
  • Molecular weight: Predicted: 85 kDa; Apparent: 93 kDa
  • Target background: CD18 (integrin ?2; ? subunit of CD11a, b, c)

Applications

  • Application: FACS, Function Blocking, Immunohistochemistry
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

References

  • Garcia-Pardo et al.1990. Journal of Immunology.144(9):3361-6.PMID: 2139453
  • Languino et al.1989. J Cell Biol.109(5):2455-62.PMID: 2530239
  • Dittel et al.1993.Blood.81(9):2272-82.PMID: 7683214