Partially inhibited T lymphocyte adhesion to intact fibronectin. However, inhibition of T lymphocyte or RD cell adhesion to fibronectin was most efficient when the inhibitory antibodies to ?4/?1 and ?5/?1 were used together.
| Inventor | Institute |
|---|---|
| Elizabeth A. Wayner, William Carter | Fred Hutchinson Cancer Center |
| Cat. #: | 161658 |
|---|---|
| Tool sub type: | Primary antibody |
| Research Fields: | Cell biology |
| Application: | FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation |
| Target: | Integrin alpha-4 (CD49d) |
| Reactivity: | Human |
| Clone: | P3E3 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Wayner et al. 1989. Journal of Cell Biology. 109(3):1321-30. PMID: 2527858 |
| Product description: | MoAb P3E3 was developed to identify a new fibronectin receptor that is identical to the integrin receptor ?4/?1. P3E3 recognized epitopes on the alpha 4 subunit and completely inhibited the adhesion of peripheral blood and cultured T lymphocytes to a 38-kD tryptic fragment of plasma fibronectin containing the carboxy-terminal Heparin II domain and part of the type III connecting segment. The ligand in type III connecting segment (IIICS ) for ?4/?1 was the CS-1 region previously defined as an adhesion site for melanoma cells. |
|---|---|
| Isotype: | IgG2b |
| Molecular weight: | 70-150kDa |
| Immunogen: | HT1080 human fibrosarcoma cell line |
| Target background: | A fibronectin receptor that is identical to the integrin receptor ?4?1. CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit ? |
|---|
| Storage conditions: | For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
Wayner et al. 1989. Journal of Cell Biology. 109(3):1321-30. PMID: 2527858 |
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