Anti-HA (H5N1) [9F4]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-HA (H5N1) [9F4]
• Research fields: Microbiology
• Clone: 9F4
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Virus
• Host: Mouse
• Application: IF ; Fn ; WB
• Description: The highly pathogenic avian influenza A subtype H5N1 virus was first isolated from geese in Guangdong province, China, in 1996. Since 2003, the H5N1 strains have caused major morbidity and mortality in poultry populations across Asia, Europe, and Africa The hemagglutinin (HA) protein is one of the two major surface glycoproteins on the envelope of influenza A virus, with 16 distinct types identified in the avian species. The HA protein is responsible for receptor binding to host cells and for viral entry and is therefore the primary target of neutralizing antibodies. In this study, the recombinant HA protein of a clade H5N1 virus was used to generate monoclonal antibodies for viral neutralization assays. The HA full-length protein was expressed in insect cells infected with baculovirus carrying the HA gene to ensure that it underwent glycosylation.
• Immunogen: HA full length that was expressed and purified from insect cells
• Isotype: IgG2b kappa
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Transfected MDCK cells

Target Details

• Target: H5N1 Influenza A virus hemagglutinin protein
• Target background: The highly pathogenic avian influenza A subtype H5N1 virus was first isolated from geese in Guangdong province, China, in 1996. Since 2003, the H5N1 strains have caused major morbidity and mortality in poultry populations across Asia, Europe, and Africa The hemagglutinin (HA) protein is one of the two major surface glycoproteins on the envelope of influenza A virus, with 16 distinct types identified in the avian species. The HA protein is responsible for receptor binding to host cells and for viral entry and is therefore the primary target of neutralizing antibodies. In this study, the recombinant HA protein of a clade H5N1 virus was used to generate monoclonal antibodies for viral neutralization assays. The HA full-length protein was expressed in insect cells infected with baculovirus carrying the HA gene to ensure that it underwent glycosylation.

Applications

• Application: IF ; Fn ; WB

Handling

• Format: Liquid
• Concentration: 1mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Mak et al. 2014. Antiviral Res. 107:76-83. PMID: 24797696.
• Chimerization and characterization of a monoclonal antibody with potent neutralizing activity across multiple influenza A H5N1 clades.
• Oh et al. 2010. J Virol. 84(16):8275-86. PMID: 20519402.
• An antibody against a novel and conserved epitope in the hemagglutinin 1 subunit neutralizes numerous H5N1 influenza viruses.