![U87 cells were transfected with siRNA against the Lon protease (siLonp1.1 and siLonp1.2) or a control sequence (siControl) or transfection reagent alone (mock) and then western blotted using anti-LONP1 [LON20/H1] and a loading control antibody (?-actin). Molecular weight markers are labelled in kilodaltons](https://cancertools.org/wp-content/uploads/3da8eca5-5e21-44ed-8a1c-11654646e43c.jpg)
Cat. #152791
Anti-LONP1 [LON20/H1]
Cat. #: 152791
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Human LON protease
Class: Monoclonal
Application: ELISA ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Helen Turley
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-LONP1 [LON20/H1]
- Alternate name: hLON, hLON ATP dependent protease, LON, LONP1, lon peptidase 1, mitochondrial, LON protease, Lon protease homolog, Lon protease like protein, Lon protease-like protein
- Research fields: Cancer;Genetics;Metabolism
- Clone: LON20/H1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 106 kDa
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Description: ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner.
- Immunogen: KLH-peptide conjugate - N-CEKDDKDAIEEKFRERLKE-C
- Isotype: IgG
- Recommended controls: U87 cells
Target Details
- Target: Human LON protease
- Molecular weight: 106 kDa
- Tissue cell line specificity: U87 cells
- Target background: ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner.
Applications
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Cameron Edward Snell PhD thesis, (2013). Mitochondrial modulators of hypoxia-related pathways in tumours. DPhil. University of Oxford.
- Snell et al., 2016. Targeting the mitochondrial Lon protease reduces aerobic glycolysis and HIF-1a stabilisation in tumour cells. Submitted for publication.
