#151295

Anti-MDM2 [SMP14]

Cat. #151295

Anti-MDM2 [SMP14]

Cat. #: 151295

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Murine Double Minute 2 protein (MDM2)

Class: Monoclonal

Application: ELISA ; IHC ; IF ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: David Lane

Institute: University of Dundee

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-MDM2 [SMP14]
  • Research fields: Cancer;Cell biology;Genetics
  • Clone: SMP 14
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; IP ; WB
  • Description: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.
  • Immunogen: Synthetic peptide CSRPSTSSRRRAISE (residues 154-167 in human MDM2) cross-linked to KLH using glutaldehyde.
  • Isotype: Ig3 Kappa
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: Breast carcinoma

Target Details

  • Target: Murine Double Minute 2 protein (MDM2)
  • Tissue cell line specificity: Breast carcinoma
  • Target background: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.

Applications

  • Application: ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Tao et al. 2013. Cell Res. 23(5):620-34. PMID: 23357851.
  • Jiang et al. 2013. Nature. 493(7434):689-93. PMID: 23334421.
  • Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
  • Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
  • Das et al. 2007. Int J Surg Pathol. 15(4):335-45. PMID: 17913939.
  • p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters.
  • Hashemi et al. 2002. Cancer Lett. 180(2):211-21. PMID: 12175554.
  • A melanoma-predisposing germline CDKN2A mutation with Fn significance for both p16 and p14ARF.
  • Zhang et al. 2001. J Biol Chem. 276(32):29702-10. PMID: 11359766.
  • Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.
  • Bttger et al. 1997. J Mol Biol. 269(5):744-56. PMID: 9223638.
  • Molecular characterization of the hdm2-p53 interaction.
  • Dowell et al. 1996. Cytopathology. 7(5):340-51. PMID: 8911758.
  • Expression of p21waf1/Cip1, MDM2 and p53 in vivo: analysis of cytological preparations.
  • Picksley et al. 1994. Oncogene. 9(9):2523-9. PMID: 8058315.
  • Immunochemical analysis of the interaction of p53 with MDM2
  • --fine mapping of the MDM2 binding site on p53 using synthetic peptides.