SAP1 Mouse

In vivo study of SAP-1 knockout; in vivo study of positive T cell selection & development

Contributors

Inventor Institute
Richard Treisman Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151554
Research Fields: Cell signaling and signal transduction;Genetics;Immunology
Target: Elk4 / SAP-1
disease: Castleman disease
Phenotype: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.
Product description: In vivo study of SAP-1 knockout; in vivo study of positive T cell selection & development
Conditional: No
Genetic background: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
Production details: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
Additional notes: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Costello et al. 2004. Nat Immunol. 5(3):289-98. PMID: 14770179.

Ternary complex factor SAP-1 is required for Erk-mediated thymocyte positive selection.

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