#151554

SAP1 Mouse

Cat. #151554

SAP1 Mouse

Cat. #: 151554

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Castleman disease

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Richard Treisman

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: SAP1 Mouse
  • Research fields: Cell signaling and signal transduction;Genetics;Immunology
  • Tool sub type: Mouse
  • Disease: Castleman disease
  • Conditional: No
  • Description: In vivo study of SAP-1 knockout; in vivo study of positive T cell selection & development
  • Genetic background: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
  • Phenotype: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.
  • Production details: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
  • Additional notes: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Elk4 / SAP-1

References

  • Costello et al. 2004. Nat Immunol. 5(3):289-98. PMID: 14770179.
  • Ternary complex factor SAP-1 is required for Erk-mediated thymocyte positive selection.