Cat. #151283
Anti-Zebrafish DeltaD [zdd2]
Cat. #: 151283
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Zebrafish DeltaD extracellular domain
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IF ; WB
Reactivity: Zebrafish
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Gavin Wright
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Zebrafish DeltaD [zdd2]
- Research fields: Cell biology;Developmental biology;Neurobiology
- Clone: zdd2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Zebrafish
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IF ; WB
- Description: Delta proteins activate Notch through a binding reaction that depends on their extracellular domains; they play a key role in the control of cell differentiation by lateral inhibition. DeltaD is widely expressed in subsets of cells in the nervous system, mesoderm, gut, and elsewhere. Deltas associate with membrane-associated scaffolding proteins MAGI1, MAGI2 and MAGI3. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA.
- Immunogen: Extracellular domain; DeltaD peptide
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
- Recommended controls: HEK293 cells transfected with deltaD; and zebrafish embryo
Target Details
- Target: Zebrafish DeltaD extracellular domain
- Tissue cell line specificity: HEK293 cells transfected with deltaD; and zebrafish embryo
- Target background: Delta proteins activate Notch through a binding reaction that depends on their extracellular domains; they play a key role in the control of cell differentiation by lateral inhibition. DeltaD is widely expressed in subsets of cells in the nervous system, mesoderm, gut, and elsewhere. Deltas associate with membrane-associated scaffolding proteins MAGI1, MAGI2 and MAGI3. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA.
Applications
- Application: ELISA ; FACS ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Sharma et al. 2019. Sci Rep. 9(1):9958. PMID: 31292468.
- Tong et al. 2019. Methods Mol Biol. 1576:273-281. PMID: 28434166.
- Gallagher et al. 2017. Dev Biol. 429(1):225-239. PMID: 28648842.
- Rothschild et al. 2013. Dev Biol. 381(1):179-88. PMID: 23747599.
- Wright et al. 2011. Development. 138(14):2947-56. PMID: 21653612.
- DeltaC and DeltaD interact as Notch ligands in the zebrafish segmentation clock.
- Fujita et al. 2011. Development. 138(9):1705-15. PMID: 21429985.
- Hong et al. 2011. PLoS One. 6(4):e19076. PMID: 21533047.
- Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
- Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.
- Wright et al. 2004. Development. 131(22):5659-69. PMID: 15509766.
- Delta proteins and MAGI proteins: an interaction of Notch ligands with intracellular scaffolding molecules and its significance for zebrafish development.
- Itoh et al. 2003. Dev Cell. 4(1):67-82. PMID: 12530964.
- Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta.
