NBF0LN6-987AL Cell Line

Sixth generation cell line derived from an ipsilateral axillary lymph node metastasis of a fifth-generation line

Contributors

Inventor Institute
Nathan E. Reticker-Flynn Stanford University
Cat. #: 162231
Cancer types: Melanoma
Organism: Mouse
Tissue: Skin (lymph node metastasis)
Gender: Female
Model: Tumorigenic cell line; syngeneic
Morphology: Mixed morphology that is predominantly spindle-shaped
Cancer Types In Detail: Melanoma
Primary citation: Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247
Product description: Sixth generation cell line derived from an ipsilateral axillary lymph node metastasis of a fifth-generation line
Gender: Female
CRISPR: No
Production details: Line was isolated from an axillary lymph node metastasis after roughly one month of growth of a LN5 tumor
Parental cell line: B16-F0
Model description: A luciferase transgene was integrated into the B16-F0 parental line, but is no loger expressed in LN metastatic progeny. WES suggests the presence of Braf and Pten mutations.
disease: Melanoma
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM (high glucose) + 10% FBS + 4mM L-glutamine (or GlutaMAX) + 1% Pen/Strep (optional)
Subculture routine: See product datasheet
Temperature: 37C
Atmosphere: 5% CO2
Storage medium: DMEM, 50% FBS, 10% DMSO
Mycoplasma free: Yes
Biosafety level: 1
References:

Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247

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