Cat. #162204
SW480 EGFP cell line GFP only transfected clone 2
Cat. #: 162204
Organism: Human
Tissue: Colon
Model: Genetically modified cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stefan Przyborski
Institute: University of Durham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SW480 EGFP cell line GFP only transfected clone 2
- Alternate name: SW480 EGFP-alone 2; SW480 GFP-alone 2; SW480 GFP2
- Parental cell: SW480
- Clone: clone 2
- Organism: Human
- Tissue: Colon
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Genetically modified cell line
- Receptors of note: No
- Description: Colon cancer cell line stably transfected with EGFP. Suitable for studying the effect of the absence of lamin A on cancer cell properties. Control for SW480/lamA and SW480-EGP emerin transfected cell lines.
- Recommended controls: Same cell line stably transfected with EGFP not tagged to any other DNA
Target Details
- Target: EGFP (Enhanced Green Fluorescent Protein)
- Target background: A large fluorescent tag for the visualisation of transfected constructs.
Handling
- Format: Frozen
- Passage number: Not known. Cells are high passage (>100) due to being a cancer cell line from a culture bank and then going through multiple passages to grow up the stable transfectants.
- Growth medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 100units/ml penicillin / 100ug/ml streptomycin
- Atmosphere: Without CO2
- Shipping conditions: Dry ice
- Storage medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 10% DMSO
- Storage conditions: Liquid Nitrogen
- Initial handling information: Continue to store at ?-70deg C. To bring up cells, defrost quickly in 37?C water bath and seed in petri dish/flask. Once cells have adhered to culture surface, change media to DMSO-free media.
- Subculture routine: Passage at 70-80% confluencey in the presence of 0.25% trypsin in 0.5mM EDTA/PBS. Incubate at 37?C, 5% Co2, humidified for 2 minutes. Split 1:4.
- Cultured in antibiotics: penicillin/streptomycin
- Characterisation tests: RT-PCR, IF and Western Blotting to confirm expression of lamin A
- Str profiling: PubMed 18714339
References
- Naomi D Willis, et al. 2008. PLoS One. 20
- 3(8):e2988. PMID: 18714339.
- Clare R Foster, et al. 2011. Nucleus. 2(5):434-43. PMID: 21983087.
