pETHSUL vector

Protein Purification; Protein Biochemistry

Contributors

Inventor Institute
Dr. Patrick Loll Drexel University
Cat. #: 156507
Tool sub type: pET
Research Fields: Other
Target: His6 tagged small ubiquitin related modfiier (SUMO) fusion target protein
Backbone size without insert: 5677
Bacterial resistance: Ampicillin
Product description: Protein Purification; Protein Biochemistry
Additional notes: The pETHSUL vector is part of a set of ligation independent cloning SUMO fusion vectors that allow for recombinant proteins to be produced with any desired sequence at the amino terminus, so long as the initial residue is not proline. This set of vectors generates affinity tagged SUMO fusion target proteins that allow the target protein to be expressed in <i> E.coli </i> and purified using subtractive affinity chromatography. The purified SUMO fusion is treated with recombinant SUMO protease (generated by pdtUD1 vector) to yield the mature target protein. The pETHSUL vector yields His6 tagged SUMO fusion proteins. Recombinant SUMO protease cleaves His6 SUMO from the purified target protein to yield the final mature protein. The pETHSUL vector was constructed based on the pET vector architecture. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Related product: pdtUD1 vector
Application notes: Protein Purification; Protein Biochemistry
References:

Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035.

Related Tools for Vectors

Cat. # Tool Name
152742 Chicken IgY precipitating protein Vector Key Info View Tool
153456 pGEX4T1-CBL-B Vector Key Info View Tool
153538 pSIEW-RUNX1-EVI1 Vector Key Info View Tool
153545 pHyg-AID* Plasmid Key Info View Tool
153547 pHyg-AID(31-114)-9myc Plasmid Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.