GN reporter cassette was generated by subcloning the nucleus and the Golgi complex reporter sequences into the pUC57-2A plasmid. This plasmid codes for the GTS-mCherry and the H2B-GFP sequences. The pUC57-GNrep was used to generate a pME-GNrep and subsequently the pEF1-GNrep plasmid; by Gateway cloning; using the pEF1-DEST as a destination vector.

Cat. #158347

pEF1-GNrep vector

Cat. #: 158347

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Contributor

Inventor: Dr Claudio Franco

Institute: Instituto de Medicina Molecular JoĂÂÄšÂo Lobo Antunes

Tool Details

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Tool name: pEF1-GNrep vector
Research fields: Cell biology

References

Barbacena et al. 2019. Genesis. 57(6):e23299. PMID: 30990965.

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