Oncolytic adenovirus encoding for CXCL10

Oncolytic adenoviruses have become commonly used viral vectors for cancer immunotherapy worldwide. Modifications in the viral genome give the potential of such viruses to serve as gene transfer vehicles and target cancer cells of malignant phenotype. Restricted curative effect with traditional cancer therapies (radiation therapy, conventional chemotherapy, etc.) is often due to the lack of […]

Contributors

Institute
University of Helsinki
Cat. #: 161948
Cancer types: Skin cancer
Research Fields: Immuno-oncology
Application: A549 cell infection and chemokine expression
Total vector size: 3609 bp
Backbone size without insert: 3235 bp
Bacterial resistance: Kanamycin
Selectable markers: kanamycin selection LB medium
Primary citation: Hamdan et al. 2021. Mol Ther Methods Clin Dev. 4;20:625-634. PMID: 33718514
Alternate name: Oncolytic adenovirus encoding for interferon ?-induced protein 10 kDa (IP-10), oncolytic adenovirus encoding for small-inducible cytokine B10
Product description: Oncolytic adenoviruses have become commonly used viral vectors for cancer immunotherapy worldwide. Modifications in the viral genome give the potential of such viruses to serve as gene transfer vehicles and target cancer cell sof malignant phenotype. Restricted curative effect with traditional cancer therapies (radiation therapy, conventional chemotherapy, etc.) is often due to the lack of tumour infiltrate immune cells. To target the absence of such effectors and facilitate their recognition of target cells, the molecular function of various signalling molecules can be investigated. Cytokines play a role in oncology since many decades by regulating the tumour-directed immune response. These molecular messengers hold the potential to switch a cold tumour containing only few immune effector cells to a hot tumour with increased tumour infiltration. The match between effector and target cells constitute a necessity for effective therapies. Therefore, cytokine encoding adenoviruses can be designed to facilitate immune infiltration based on their role, mainly, in T-cell attraction. CXCL10 belongs to the subgroup of cytokines known as chemokines. These small molecular weighted proteins are known to direct cell movement towards a gradient in various tissue types. Bronger and colleagues investigated in the effect of CXCL10 to facilitate lymphocyte infiltration into the tumour. They showed such association through binding to the cognate chemokine receptor, CXCR3, preferentially present on CD8 T-cells, Th1 T-cells, NK (T-cells), dendritic cells and monocytes.
Application: A549 cell infection and chemokine expression
References:

Hamdan et al. 2021. Mol Ther Methods Clin Dev. 4

20:625-634. PMID: 33718514

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