pJEK1 Alpha-Synuclein Vector

pJEK1 is a derivative of pET15b with the human alpha-synuclein open reading frame cloned in via the Ndel and BamHI restriction sites. In this construct alpha-synuclein is expressed as a fusion protein with a six His tag. 2004 FASEB paper: this alpha-synuclein protein available here is cited as source (2) in the Methods section.

Contributors

Inventor Institute
Dr Fiona Benson Lancaster University
Cat. #: 152046
Research Fields: Neurobiology
Target: Alpha-synuclein
Product description: pJEK1 is a derivative of pET15b with the human alpha-synuclein open reading frame cloned in via the Ndel and BamHI restriction sites. In this construct alpha-synuclein is expressed as a fusion protein with a six His tag. 2004 FASEB paper: this alpha-synuclein protein available here is cited as source (2) in the Methods section.
Additional notes: Alpha-synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
Application notes: pJEK1 is a derivative of pET15b with the human alpha-synuclein open reading frame cloned in via the Ndel and BamHI restriction sites. In this construct alpha-synuclein is expressed as a fusion protein with a six His tag. 2004 FASEB paper: this alpha-synuclein protein available here is cited as source (2) in the Methods section.
References:

El-Agnaf et al. 2004. FASEB J. 18(11):1315-7. PMID: 15180968.

A strategy for designing inhibitors of alpha-synuclein aggregation and toxicity as a novel treatment for Parkinson's disease and related disorders.

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