SW48-PLCG1

For stable PLCG1 overexpression (SW48-PLCG1), SW48 cells were infected with PLCG1 lentiviral activation particles (Santa Cruz Biotechnology). Selection of stable clones started 2 days after infection with a mix of 2 ?g/mL Puromycin, 50 mg/mL Hygromicyn B, and 10 mg/mL Blasticidin.

Contributors

Inventor Institute
Marta Martins Instituto de Medicina Molecular João Lobo Antunes
Cat. #: 161945
Cancer types: Colorectal cancer
Research Fields: Cancer
Organism: Human
Tissue: Epithelial
Gender: Male
Model: Over-expression
Growth properties: A subcultivation ratio of 1:4 to 1:6 is recommended, 1 to 2 times per week.
Primary citation: Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600
Product description: For stable PLCG1 overexpression (SW48-PLCG1), SW48 cells were infected with PLCG1 lentiviral activation particles (Santa Cruz Biotechnology). Selection of stable clones started 2 days after infection with a mix of 2 ?g/mL Puromycin, 50 mg/mL Hygromicyn B, and 10 mg/mL Blasticidin.
Gender: Male
CRISPR: No
Parental cell line: SW480
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 10% FBS, 5% CO2
Recommended controls: shRNA scrambled control
Biosafety level: 1
References:

Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600

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