MEF Raf1 FF/FF KI Cell Line

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The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
SKU: 151629
Product description: The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.
Alternate name: Raf-1FF/FF MEF; MEF Raf1 FF/FF KI
Conditional: Yes
Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Cellosaurus ID: CVCL_6D90
Parental cell line: See the Raf KI FF knockout mouse that these cells were derived from in related products.
Disease: Cancer
Cat. #: 151629
Provenance and Ownership: The MEF Raf1 FF/FF KI Cell Line is owned by Cancer Research UK. CancerTools supplies the MEF Raf1 FF/FF KI Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MEF Raf1 FF/FF KI Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Organism: Mouse
Tissue: Embryo
Model: Knock-In
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
Mycoplasma free: Yes
Biosafety level: 1
References:

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

MEK kinase activity is not necessary for Raf-1 function.

Images

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MEF Raf1 FF/FF KI Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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