| Inventor |
|---|
| Pranab K Das |
| SKU: | 154104 |
|---|---|
| Product description: | PiebaldismÄÂ? is a rareÄÂ? autosomal dominantÄÂ? disorder ofÄÂ? melanocyteÄÂ? development. Common characteristics include a congenital whiteÄÂ? forelock, scattered normal pigmented and hypo pigmentedÄÂ? maculesÄÂ? and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung’s disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research |
| Gender: | Male |
| CRISPR: | Yes |
| Conditional: | No |
| Production details: | Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418). |
| Parental cell line: | Lesional skin of piebaldism patient |
| Disease: | Piebaldism;Piebaldism |
| Cat. #: | 154104 |
|---|---|
| Tool sub type: | 10XUAS IVS p10 |
| Unit size: | 1×10^6 cells / vial |
| Organism: | Human |
| Gender: | Male |
| Model: | Primary line |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418) |
| Mycoplasma free: | Yes |
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