MDA-MB-231/EGFP_Cytochrome c

Stably expressing single cell clones with homogeneous expression of Cytochrome c_EGFP were isolated for high resolution imaging. The expression and functionality of exogenous Cytochrome c_EGFP was confirmed through fluorescence microscopy by its release upon apoptotic stimulus.

Contributors

Inventor Institute
T.R. Santhosh Kumar Rajiv Gandhi Centre for Biotechnology
Cat. #: 161147
Cancer types: Breast cancer
Research Fields: Cancer;Disease (not cancer);Metabolism
Organism: Human
Tissue: Breast
Gender: Female
Model: Transgenic
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Breast cancer
Primary citation: Srinivas KP, et.al. PMID:26992219
Product description: The product is a human breast carcinoma cell line MDA-MB-231, transfected to stably express the protein cytochrome c (cyt-c) tagged with EGFP. The EGFP-tag enables easy identification of the cyt-c release in live cells upon apoptotic induction. The cell line can be utilized for studying the apoptosis process and to screen of potential candidate drugs that induce apoptosis.
Gender: Female
CRISPR: No
Conditional: No
Parental cell line: MDA-MB-231
Disease: Cancer
Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or Leibovitz's L15 with 2mM L-Glutamine and 10% Fetal Bovine serum.
Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: Growth medium + 10% DMSO
References:

Srinivas KP, et.al. PMID:26992219

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