H357 Cell Line

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A human oral squamous cell carcinoma model used to study TGF-β signalling, epithelial-to-mesenchymal transition and head and neck cancer biology. Save 30% on this cell line this autumn. Use code ACA26CELL at checkout.

Contributors

Inventor Institute
Stephen Prime University of Bristol
SKU: 153422
Product description: H357 was established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. STNMP stage I, well differentiated, node negative tumour. Mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line: codon: 13, G to A, codon 61, A-G is under investigation. This cell line is highly responsive to TGF beta and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF beta1. Fahey et al (1996). Tumourigenic in athymic nude mice on subcutaneous injection and non-tumorigenic on orthotopic injection. Haplotype information: A*02,A*31; B*40,B*44; Cw*03,Cw*05
Alternate name: H-357; h357
Conditional: Yes
Production details: Established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient.
Cellosaurus ID: CVCL_2462
Receptors of note: TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
Disease: Cancer
Cat. #: 153422
Provenance and Ownership: The H357 Cell Line is owned by Cancer Research UK. CancerTools supplies the H357 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing H357 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Tissue: Tongue
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Head and neck cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
Subculture routine: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density of 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.
References:

Fahey et al. 1996. Br J Cancer. 74(7):1074-80. PMID: 8855977.

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31(2):136-143. PMID: 7633286.

Prime et al. 1994. Int J Cancer. 56(3):406-412. PMID: 7508893.

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.

Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-67. PMID: 8180579.

Prime et al. 1990. J Pathol. 160(3):259-269. PMID: 1692339.

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