H400 Cell Line

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Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm – 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour.

Contributors

Inventor Institute
Stephen Prime University of Bristol
SKU: 153424
Product description: Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm – 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour. This cell line is highly responsive to TGF-beta. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
Alternate name: h400; H-400
Gender: Female
CRISPR: No
Conditional: No
Additional notes: Haplotype information: A*11,A*29; B*07,B*15; Cw*03,Cw*15
Cellosaurus ID: CVCL_2464
Model description: Mutant p53, codon 283 exon 8, C to G wild type K-, N- and Ha-ras. Non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth.
Disease: Cancer
Cat. #: 153424
Provenance and Ownership: The H400 Cell Line is owned by Cancer Research UK. CancerTools supplies the H400 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing H400 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: The alveolar process of the maxilla
Gender: Female
Model: Tumour cell line
Growth properties: Adherent
Primary citation: Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Subculture routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37C. Suggested seeding density 5 x 1000 cells/cm2. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286

Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215

Yeudall et.al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579

Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339

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