H314 Cell Line

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H314 Cell Line is established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of an 82 year-old male patient.

Contributors

Inventor Institute
Stephen Prime University of Bristol
SKU: 153421
Product description: Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of an 82 year-old male patient. STNMP stage II, moderately differentiated, node positive tumour. Mutant p53, codon 176 exon 5, G to T and 373 exon 11, A to G; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice on subcutaneous injection; on injection into the floor of the mouth, cells spread to lymphatics and lymph nodes. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome. Explore more human oral SCC cell lines from the same inventor including: H157, H376, H357, H400 and H103.
Alternate name: H-314; h314; SCC-HN; HNSCC; HN-SCC
Conditional: Yes
Cellosaurus ID: CVCL_2460
Disease: Cancer
Cat. #: 153421
Provenance and Ownership: The H314 Cell Line is owned by Cancer Research UK. CancerTools supplies the H314 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing H314 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Tissue: Oral Cavity
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Head and neck cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%), approximately every 6-7 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 7 x 1000 cells/cm2. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
References:

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-143. PMID: 7633286.

Prime et al. 1994. Int J Cancer. 56(3):406-412. PMID: 7508893.

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.

Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-67. PMID: 8180579.

Prime et al. 1990. J Pathol. 160(3):259-269. PMID: 1692339.

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