H103 Cell Line

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The H103 Cell Line was established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient.

Contributors

Inventor	Institute
Stephen Prime	University of Bristol

SKU:	153419
Product description:	Established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Alternate name:	h103; H-103
Cellosaurus ID:	CVCL_2457
Disease:	Cancer

Cat. #:	153419
Provenance and Ownership:	The H103 Cell Line is owned by Cancer Research UK. CancerTools supplies the H103 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing H103 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size:	1×10^6 cells / vial
Research Fields:	Cancer
Tissue:	Tongue
Model:	Tumourigenic cell line
Growth properties:	Adherent

Format:	Frozen
Shipping conditions:	Dry ice
Growth medium:	Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?Â°C. Suggested seeding density 5 x 1000 cells/cm?Â . Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydroco…

References:	Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-143. PMID: 7633286. Prime et al. 1994. Int J Cancer. 56(3):406-412. PMID: 7508893. Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215. Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-67. PMID: 8180579. Prime et al. 1990. J Pathol. 160(3):259-269. PMID: 1692339.

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