CD6-RFP Jurkat cell line

Activation of the T cell receptor (TCR) on the T cell through ligation with antigen-MHC complex of an antigen-presenting cell (APC) is an essential process in the activation of T cells and induction of the subsequent adaptive immune response. Upon activation, the TCR, together with its associated co-receptor CD3 complex, assembles in signaling microclusters that […]

Contributors

Inventor Institute
Alessandra Cambi Radboud University Medical Center
Cat. #: 160713
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology
Organism: Human
Tissue: Blood
Cancer Types In Detail: Acute T cell Leukemia
Alternate name: CD6
Product description: Activation of the T cell receptor (TCR) on the T cell through ligation with antigen-MHC complex of an antigen-presenting cell (APC) is an essential process in the activation of T cells and induction of the subsequent adaptive immune response. Upon activation, the TCR, together with its associated co-receptor CD3 complex, assembles in signaling microclusters that are transported to the center of the organizational structure at the T cell-APC interface termed the immunological synapse (IS). During IS formation, local cell surface receptors and associated intracellular molecules are reorganized, ultimately creating the typical bull's eye-shaped pattern of the IS. CD6 is a surface glycoprotein receptor, which has been previously shown to associate with CD3 and co-localize to the center of the IS in static conditions or stable T cell-APC contacts.
Conditional: No
Production details: Jurkat cell lines stably expressing CD6-RFP were obtained by electroporation using the Neon Transfection System for Electroporation (Invitrogen) according to the manufacturer's guidelines. Shortly, 5*105 Jurkat cells were transfected at 1325 Volt (10 ms, 3 pulses) with 3 ÎĚ?Ÿg of DNA in 100 ÎĚ?Ÿl Resuspension buffer. After transfection cells were seeded in 2 ml of 1640 RPMI with 10% FCS and 1% U-glut. Antibiotics were added after 3 h. Stable cell lines were sorted on RFP expression on…
Parental cell line: Jurkat E6.1
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: 1640 RPMI (PAA) supplemented with 10% Fetal Calf Serum, 1 mM Ultra-glutamine and antibiotics (100 U/ml penicillin, 100 ?g/ml streptomycin and 0.25 ?g/ml amphotericin B). 500 ng/ml geneticin.
References:

Meddens et al. 2018. Front Immunol. 9:2333. PMID: 30356797.

Images

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BICR 10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
CD6 and TCR/CD3 in Jurkat T cells co-localize upon ligation through micropatterned antibody spots. (A

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