MEF Trex1 KO Cell Line

Trex1 KO MEF cell line is a spontaneously transformed mouse embryonic fibroblast (MEF) cell line. It allows the study of novel interconnections.

Contributors

Inventor Institute
Deborah Barnes Cancer Research UK, London Research Insitute: Clare Hall Laboratories
Cat. #: 151558
Unit size: 1×10^6 cells / vial
Research Fields: Genetics;Immunology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Defective G1/S transition, increased cell doubling time; chronic ATM-dependent checkpoint activation even in the absence of exogenous stress; persistant ssDNA polynucleotide species generated in S phase that accumulates in the cytoplasm.
Product description: The Trex1 KO MEF cell line is a spontaneously transformed mouse embryonic fibroblast (MEF) cell line. It allows the study of novel interconnections between DNA replication, DNA damage checkpoint signalling and antiviral-like autoimmune responses. Recapitulates cellular phenotype of Trex1-deficient Aicardi-Goutieres syndrome. Spontaneously transformed clone derived following repeated passaging in culture by standard techniques of primary MEFs from Trex1-/- knockout mouse
Conditional: Yes
Production details: MEF from Trex1-/- knockout mouse
Cellosaurus ID: CVCL_5A06
Disease: Trex1-deficient Aicardi-Goutieres syndrome
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
References:

Yang et al. 2007. Cell. 131(5):873-86. PMID: 18045533.

Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease.

Morita et al. 2004. Mol Cell Biol. 24(15):6719-27. PMID: 15254239.

Gene-targeted mice lacking the Trex1 (DNase III) 3'–>5' DNA exonuclease develop inflammatory myocarditis.

Images

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MEF Trex1 KO Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC).

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